胡文军; 傅庭治; 曹幼琴
南京大学生物科学与技术系
【中文摘要】 本研究报导了液体深层培养金针菇（ＦｌａｍｍｕｌｉｎａｖｅｌｕｔｉｐｅｓＮＪ９６０１）的富硒能力和生物转化。在添加５～１２５（×１０－６）硒的范围内，菌丝体富硒的范围为８．２～９０．２（×１０－６）；添加２０（×１０－６）时，菌丝体吸收率最高，为对照的１８４％，此时菌体生长、可溶性蛋白质、氨基酸、多糖等含量均高于对照组。无机硒经菌体吸收、代谢、转化，其有机化程度在７５％以上，分布在硒蛋白（占６４．１％），硒多糖（占１１．２％）。用反相高效液相色谱法定量分析硒蛋氨酸含量。用富硒金针菇粉和剩余培养液分别饲养蚯蚓，其产量可提高３０％以上。从饲养的蚯蚓提取分离获得的蚓激酶产率提高２０％。开辟了富硒金针菇综合利用的新途径
【英文摘要】 In this paper, we report the ability of Flammulina uelutipes NJ9601, which cultivated in deep-liquid culture, to gather selenium and its biotransformation. When the selenium added to the culture is 5～125 (×10 -6 ), selenium contained in the hyphostroma is 8 2～90 2(×10 -6 ). When the selenium added to the culture is 20(×10 -6 ), the hyphostroma have the most selemium, which is 184% times more than that in the control. The dissoluble proteins, amino acids, polysaccharides in the hyphostroma wrer all more than those in the control groups. About 75% of nonorganic selenium that absorbed into the hyphostroma were transformed into organic selenium, which can be found in Se-proteins (64.1%), Se-polysaccharides(11.2%) and so on. The contents of Se-Met were quantitatively measured by reverse-high performance liquid chromotography. Applying the powders of Flammulina velutipes rich in selenium and the second-hand liquid cultures to earthworms, we found that the yield of earthworms could increase b...更多y 30% or more. And the yield of the fibrinolytic enzymes extracted from earthworms can increase by 20% or more. In this way, we open a new, comprehensive way to utilize Flammulina velutipes rich in selenium. 还原
【中文关键词】 金针菇; 硒蛋氨酸; 富硒
【英文关键词】 Flammulina velutipes; Se-Met; Se-concentration
【文献出处】 中国食用菌,EDIBLE FUNGI OF CHINA,编辑部邮箱,1997年03期 【DOI】CNKI:SUN:ZSYJ.0.1997-03-019