FU Li-zhong 1; 2; WEI Hai-long 1; 2; LI Hai-bo 1; 2; WU Qing-qi 1; 2; WU Da-feng 2; WU Xue-qian 1; 2* 1 Biotechnology Research Institute; Zhejiang Academy of Forestry Science; Hangzhou 310023; China; 2Lishui Edible Fungi Research and Development Center; Zhejiang Essence Fungi Development Co.; Ltd; Lishui; Zhejiang 323000; China
【英文摘要】 A stable Sequence-Related Amplified Polymorphism (SRAP) molecular marker system for Lentinula edodes has been developed and optimized using genomic DNA extracted from fungal mycelium with sodium dodecyl sulfate-cetyltrimethylammonium bromide (SDS-CTAB). A large number of amplification products and good reproducibility was achieved using a 20 μL reaction system consisting of 2 μL 10×Reaction Buffer(containing 1.5 mM Mg 2+), 25 ng genomic DNA, 200 μM dNTP mixture, 1.0 mM Mg 2+, 0.4 μM forward and reverse primer (Me1 and Em16), 1.5 U Taq polymerase, and ddH_ 2O to volume.
【英文关键词】 Lentinula edodes; Molecular marker; Optimization of amplification protocol; SRAP
【基金】Supported by Key Technologies R&D Programs of Zhejiang Province(No.2005C22057and2004C22032),andKey Programof Zhejiang Academy of Forestry Science(No.2006F11003)
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2006年04期 【DOI】CNKI:SUN:SYJB.0.2006-04-003