【作者】 曹育博; 刘天翔; 周巍巍; 高玉千; 戚元成; 申进文; 邱立友;
【Author】 CAO Yubo,LIU Tianxiang,ZHOU Weiwei,GAO Yuqian,QI Yuancheng,SHEN Jinwen,QIU Liyou College of Life Sciences,Henan Agricultural University,Key Laboratory of Enzyme Engineering of Agricultural Microbiology,Ministry of Agriculture,Zhengzhou,Henan 450002,China
【机构】 河南农业大学生命科学学院农业部农业微生物酶工程重点实验室;
【摘要】 以双孢蘑菇(Agaricus bisporus)2793为试材,采用基因枪法对其菌褶进行遗传转化,相对转化率为2.69%,转化子经PCR检测表明,外源潮霉素B抗性基因已经被转入到菌丝体中。
【Abstract】 Transformation of Agaricus bisporus by particle bombardment of fruit body gill tissue was confirmed using the hygromycin B gene as a selective marker.PCR amplification revealed that DNA containing the resistance marker had been inserted into fungal hyphae with a transformation efficiency of 2.69%.
【关键词】 双孢蘑菇; 基因枪; 遗传转化; 潮霉素B抗性;
【Key words】 Agaricus bisporus; particle bombardment; genetic transformation; hygromycin B resistance;
【基金】 河南省科技攻关项目(编号:082102150048)的部分研究内容
【分类号】S646.11