汪中文; 林芳灿
华中农业大学!武汉430070; 华中农业大学!武汉430070
【中文摘要】 以在湖北省无栽培菌株抱子传播的香菇（Lentinula edodes）自然发生地随机采集的32个野生香菇菌株为材料,用原生质体单核体和孢子单核体配对相结合的方法分析鉴定样本中的交配型因子,并用统计学方法分析了菌丝原生质体单核体、孢子单核体的交配型比例及样本中特异交配型因子的分布频率。在大部分菌株中,菌丝原生质体单核体的两种交配型呈预期的1:1之比,孢子单核体的四种交配型亦呈预期的1:1:1:1之比.从样本中鉴定出52个特异的A因子及55个特异的B因子,而且这一系列特异性的A因子和B因子都呈随机分布。与栽培菌株相比,野生菌株的交配型因子重复频率要低得多,表明香菇自然种群经过长期遗传变异积累了丰富的种质资源。在今后的香菇菌株遗传改良工作中.更多地引人野生种质资源以丰富育种材料的遗传多样性具有重要意义。
【英文摘要】 Mating type factors in 32 strains of Lentinula edodes collected from mountainous region of Hubei Province without dissemination of spores of cultivated strains were determined by the mating test between spore monokaryons and protoplast monokaryons derived from binuclear mycelia. In addition, analyses of the mating type ratio of protoplast monokaryons and spore monokaryons and the distribution frequency of specific mating factors in the samples were made with statistical methods. The results indicated that both the expected 1:1 ratio of mating types of protoplast monokaryons and the expected 1:1:1:1 ratio of mating types of spore monokaryons were obtained in most of the strains. 52 distinct A factors and 55 distinct B factors were determined from the sample and the series of both the specific A factors and B factors were randomly distributed. The replication frequency of mating factor in wild strains was much lower than that in cultivated strains. It meant that there was abundant germpl...更多asm resource in the natural population of Lentinula edodes. Therefore, it was important that the wild strains be served as the primary breeding materials for improving genetic variability of commercial strains of Lentinula edodes. 还原
【中文关键词】 香菇; 交配型因子; 自然种群; 种质资源
【英文关键词】 Lentinula edodes; Mating type factor; Natural population; Germplasm resource
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,1996年04期 【DOI】CNKI:SUN:SYJB.0.1996-04-001