宋思扬; 曾伟; 陈融; 王泽生; 苏文金
厦门大学肿瘤细胞工程国家专业实验室!厦门361005; 厦门大学肿瘤细胞工程国家专业实验室!厦门361005福建省轻工业研究所; 福州350005; 厦门大学肿瘤细胞工程国家专业实验室!厦门361005; 福建省轻工业研究所!福州350005; 厦门大学肿瘤细胞工程国家专业实验室!厦门361005
【中文摘要】 利用 2 0个十碱基随机引物 ,在基因组水平上对双孢蘑菇 (Agaricusbisporus)的优质低产型 (G型 )及低质高产型 (H型 )菌株进行了RAPD差异显示 ,获得一个与双孢蘑菇子实体品质相关的大小约为 2 0 0 0bp的差异DNA片段 ,将其克隆 ,并应用点杂交及RFLP技术对其区分两类菌株的有效性进行了检验。这为进一步建立特异性探针或PCR引物 ,从分子水平上提高双孢蘑菇菌种性状早期预测的准确性奠定了基础
【英文摘要】 With 20 ten-mers random primers, the genomic differences between two types of Agaricus bisporus strain-good quality with low yield (G type) vs. poor quality with high yield (H type), were displayed by Random Amplified Polymorphic DNA (RAPD) technique. A 2000bp specific DNA fragment correlated to the quality of Agaricus bisporus fruitbody was obtained and cloned. Then dot hybridization and RFLP technique were used to determine the effectiveness of distinguishing two types of A. bisporus strains. And it was considered that our study had laid a foundation for further establishing a specific probe or PCR primer and for improving the accuracy of early predicting the characteristics of A. bisporus strains at molecular level.
【中文关键词】 双孢蘑菇; RAPD; 子实体品质; DNA克隆; RFLP
【英文关键词】 Agaricus bisporus; RAPD; Fruitbody; Cloning; RFLP
【基金】福建省自然科学基金资助项目!(C982 0 0 0 5 )
【文献出处】 食用菌学报,ACTA EDULIS FUNGI,编辑部邮箱,2000年03期 【DOI】CNKI:SUN:SYJB.0.2000-03-002