范雷法; 潘慧娟; Soccol CR; Pamdey A
浙江省农业科学院园艺所; 浙江省农业科学院园艺所; Laboratory of Biotechnological Process; Federal University of Parana; Biotechnology Division; Regional Research Laboratory 杭州310021 ; 杭州310021 ; Curitiba-PR; 81531-970 Brazil ; Trivandrum-695 019; India
【中文摘要】 通过深层发酵培养姬松茸菌丝体,滤取菌丝体后获得菌丝体培养液,经沉淀离心收集其胞外粗多糖,再用蒸馏水溶解,取可溶性部分。对接种S 180的小白鼠24h后腹腔注射给药,连续10d,剂量为10mg kg·d。5周后剥取瘤块,与对照组比较瘤重。结果发现,姬松茸深层发酵生产的胞外多糖对小鼠接种的腹水瘤S 180有较强的抗性,肿瘤抑制率达到44.53%,完全抑制率达到50%。
【英文摘要】 Ab-EPS was produced by submerged fermentation and extracted from the liquid media by ethanol precipitation and centrifugation. After removing the insoluble part in water, the Ab-EPS was applied to mice inoculated with tumor cell of S-180 24h later by intraperitoneal injection for 10 consecutive days at the dose of 10 mg/kg·d. Five weeks later, the tumor weight was evaluated and compared with that of the control group. Ab-EPS showed a higher antitumor activity against S-180 grafted in mice with the tumor inhibition rate being 44.53% and the complete inhibition rate being 50%.
【中文关键词】 姬松茸; 胞外多糖; 抗肿瘤活性; S180
【英文关键词】 Agaricus blazei; Exopolysaccharide; Antitumor activity; S-180
【基金】巴西科技部资助项目"姬松茸胞外多糖的优化生产和抗肿瘤研究"(CNPq:140165 2000-2)的部分内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2004年02期 【DOI】CNKI:SUN:SYJB.0.2004-02-010