贾定洪; 郑林用; 王波; 郭勇; 甘炳成; 黄忠乾; 彭卫红
四川省农业科学院土壤肥料研究所
【中文摘要】 以7个栽培菌株为参照,采用ISSR分子标记技术对5个野生木耳属菌株进行分析,使用NTSYSpc2.1生物软件对12个供试菌株进行聚类分析,构建系统树。试验筛选出了11个扩增谱带清晰、多态性好的ISSR引物,共扩增出78条清晰易辨的多态性谱带。聚类分析结果表明,利用ISSR技术可将全部供试材料区分开,遗传相似系数变异范围为0.1080.822,在相似系数为0.51时,12个菌株聚为6个群,其中5个野生菌株各自聚为不同类群,遗传差异较大。
【英文摘要】 Five wild-type Auricularia strains were identified by ISSR(inter-simple sequence repeat)methodology using seven cultivated Auricularia strains as controls.Eleven ISSR primers,selected from a total of 60 test ISSR primers,amplified 78 distinct polymorphic DNA bands from the 12 Auricularia strains.ISSR similarity coefficients ranged from 0.108 to 0.822,and a dendrogram based on UPGMA(unweighted pair group method with arithmetic average) cluster analysis using these values revealed that the 12 strains were clustered into six groups(similarity coefficient 0.51).The five wild-type strains clustered into five different groups,and the most distinct strain genetically was the wild-type strain,Ausm(similarity coefficient 0.198).
【中文关键词】 木耳属; ISSR; 遗传鉴定
【英文关键词】 Auricularia; inter-simple sequence repeats; genetic identification
【基金】四川省财政育种工程资助项目“食用菌新品种选育及新材料新方法研究”(编号:2006YZCC09-024);; 四川省育种攻关资助项目“优质丰产黑木耳新品种选育”(编号:YZGG06-04-01)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2008年03期 【DOI】CNKI:SUN:SYJB.0.2008-03-002