沈学香; 尚晓冬; 汪虹; 谭琦
农业部应用真菌资源与利用重点开放实验室; 上海市食用菌工程技术研究中心; 上海市农业遗传育种重点实验室; 上海市农业科学院食用菌研究所
【中文摘要】 用对峙培养技术在PDA平板上对10株香菇菌株的营养体不亲合性进行了研究。结果表明,供试菌株的营养体不亲合性表现可分为两种不亲合类型和一种亲合类型。在2次重复实验中表现完全一致的有48对组合,占87.3%;表现不同但仍可认为是亲合类型而不影响结果性质的有4例,占7.3%,分别是135×66、9015×66、9015×7402和Cr04×野生50;表现出相反的结果,影响亲合性判定的有3例占5.4%,分别是Cr04×26、Cr04×申香10号和26×苏香。11株供试香菇菌株间营养体不亲合性的表现以不亲合类型为主,第一种和第二种不亲合类型分别出现86次(78.2%)和13次(11.8%);亲合类型(第三种类型)较少,有11次(10%)。135、939和9015间彼此亲合,它们与其它菌株均表现不亲合。申香10号与26亲合,2菌株与除Cr04和苏香以外的菌株均不亲合。
【英文摘要】 Somatic incompatibility (SI) between ten cultivars and one wild-type strain of Lentinula edodes was determined from mycelial growth confrontation reactions on potato dextrose agar plates. Two types of SI reactions were observed, together with one type of somatic compatibility (SC) reaction. Identical SI or SC reactions were recorded in duplicate confrontation tests between 48 pairs of L. edodes strains yielded, accounting for 87.3% of the total 110 test pairings. Different SI reactions were observed in duplicate pairings between strains 135 and 66, 9015 and 66, 9015 and 7402, and Cr04 and 50 (7.3% of the total). The opposite reaction (from SI to SC or vice versa) was observed in three sets of duplicate confrontation tests (5.4% of the total pairings) involving strains Cr04 and 26, Cr04 and Shenxiang 10, and 26 and Suxiang. The two SI reaction types were the main responses between the eleven test strains and accounted for 90% of the total. Strains 9015, 135 and 939 were somatically com...更多patible with each other but somatic incompatible with the remaining strains. Strains Shenxiang 10 and 26 were somatically compatible with each other but somatically incompatible with all the other strains except Cr04 and Suxiang. 还原
【中文关键词】 香菇; 营养体不亲合性; 拮抗反应
【英文关键词】 Lentinula edodes; somatic incompatibility; antagonism reaetion
【基金】上海市农委科技兴农重点攻关“香菇纯‘自交系’育种技术的应用研究”的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2009年02期 【DOI】CNKI:SUN:SYJB.0.2009-02-001