江玉姬; 王秀全; 刘维侠; 谢宝贵; 朱坚
福建农林大学菌物研究中心; 福建农林大学食品科学学院; 中国热带农业科学院橡胶研究所; 福建农林大学园艺学院
【中文摘要】 从29株金针菇(Flammulina velutipes)中经单孢分离获得的单核菌株,两两配对后,在显微镜下观察锁状联合,配对结果将金针菇的A因子确定到10个,B因子确定到8个。13个黄色菌株的不亲和性因子包含了从A1到A9,B1到B8;而16个白色菌株的不亲和性因子大多为A2A3B2B3类型。有24个金针菇菌株获得的单核菌丝有4种交配型且符合1∶1∶1∶1比例,其它菌株获得的单核菌丝交配型与理论数据有偏离。
【英文摘要】 Monokaryons isolated from single spores of 29 Flammulina velutipes strains were mated with each other and the formation of dikaryons determined by the presence of clamp connections.Ten A and eight B mating factors were identified.Mating types of 13 yellow strains consisted of different combinations of A1 to A9 and B1 to B8 mating factors,whereas the majority of white strains were of the A2A3B2B3 mating type.The four mating types observed among the monokaryons derived from 24 strains were present in a 1∶1∶1∶1 ratio whereas mating types in monokaryons derived from the remaining strains deviated from the theoretical distribution.
【中文关键词】 金针菇; 不亲和性因子; 遗传关系; 交配型
【英文关键词】 Flammulina velutipes; incompatibility factors; genetic relationship; mating type
【基金】福建省自然科学基金资助项目“金针菇遗传连锁群构建的研究”(编号:2006J055);; 福建省科技厅资助项目“金针菇工厂化栽培专用品种的选育与示范”(编号:2007N0003);; 福州市科技局计划项目“金针菇工厂周年生产智能化控制技术的研究与示范”[编号:榕科(2007)109号]的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2009年04期 【DOI】CNKI:SUN:SYJB.0.2009-04-003