【作者】 王淑蕾; 梁敬钰; 唐庆九; 刘艳芳; 周帅; 杨焱; 张劲松;
【Author】 WANG Shulei1,2,LIANG Jingyu2,TANG Qingjiu1,LIU Yanfang1,ZHOU Shuai1,YANG Yan1,ZHANG Jingsong1 1National Engineering Research Center of Edible Fungi;Shanghai Key Laboratory of Agricultural Genetics and Breeding;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China;2 Traditional Chinese Medicine College,China Pharmaceutical University,Nanjing,Jiangsu 210009,China
【机构】 农业部南方食用菌资源利用重点实验室国家食用菌工程技术研究中心上海市农业遗传育种重点开放实验室上海市农业科学院食用菌研究所; 中国药科大学中药学院;
【摘要】 建立1种运用高效液相色谱(HPLC)测定香菇(Lentinula edodes)中香菇嘌呤含量的方法。优化后的色谱条件为:Ultimate AQ-C18柱(5μm,4.6 mm×250 mm),流动相为甲醇-磷酸盐缓冲液(7∶93,pH 4.67)等度洗脱,流速1 mL/min,柱温30℃,检测波长259 nm,进样量10μL。研究结果发现该方法准确、灵敏、重现性好,适用于香菇嘌呤的定量分析。
【Abstract】 A simple and sensitive HPLC-based method for the accurate determination of eritadenine in Lentinula edodes fruit bodies is described.Optimization of sample work-up procedures showed that the efficiency of eritadenine extraction was highest when powdered fruit bodies were suspended in 5%(w/v) ethanol and subjected to ultrasound for 30 min using a 1∶100 sample-solvent ratio.Optimal separation of eritadenine in the extracts was achieved by loading 10 μL samples on to an Ultimate AQ-C18 column(5 μm,...
【关键词】 香菇; 香菇嘌呤; HPLC;
【Key words】 Lentinula edodes; eritadenine; HPLC;
【基金】 上海市科技兴农重点攻关项目[编号:沪农科攻字(2009)第2-2号]的部分研究内容~~
【分类号】R151.3