香菇正反双单杂交后代的遗传分析
叶明 潘迎捷 马桂荣 陈咏萱
摘 要：选用香菇的杂交菌株农1与野生株Q进行正反双单杂交,得到6个杂交后代。结果表明：3个正交菌株与3个反交菌株在酯酶同工酶与DNA水平上具有极高的相似性，而对杀菌剂和温度的敏感性显示了明显的遗传差异，且农艺性状遗传差异也十分显著。正反杂交菌株在核基因相同情况下的遗传差异应主要归于细胞质差异。本研究表明，香菇双单杂交后代是同质异核体。
关键词：香菇, 正反双-单杂交, 酯酶同工酶, RAPD, 敏感性, 农艺性状, 同质异核体
分类号：Q939.96 文献标识码：A
文章编号：1007-3515（2001）01-0094-0099
GENETIC ANALYSIS OF THE HYBRIDS OF THE POSITIVE AND NEGATIVE DI-MON MATING IN LENTINULA EDODES.
YE Ming（State Key Lab.of Microbiol.Technol.,Shandong Univ.,Jinan 250100;Department of Biological and Chemical Engineering,Anhui College of Mechanical and Electrical Engineering ,Wuhu 241000）
PAN Ying-Jie（Laboratory of Genetic and Breeding of Mushroom,Agricultural Ministry of China;Shanghai 201106）
MA Gui-Rong（State Key Lab.of Microbiol.Technol.,Shandong Univ.,Jinan 250100）
CHEN Yong-Xuan（Department of Microbiology, Nanjing Agricultural University, Nanjing 210095)）
Abstract：The positive and negative di-mon mating of a crossed strain Nong1 and a wild strain Q from Lentinula edodes were carried out and six hybrids were obtained. The results show that three strains of the positive cross and three strains of the negative cross exist high similarity on the esterase isoenzyme and DNA molecular level. However, they show distinct genetic differences on sensitivity to prochlorac (germicide)and temperature.The agronomic characters of the hybrids are also evidently diffrenet . Clearly, the genetic differences of hybrids of the positive and negative di-mon mating should be attributed to the differences of cytoplasm. This study indicate that the positive and negative di-mon mating hybrids are homogenous heterokaryons in Lentinula edodes .
Keywords：Lentinula edodes, The positive and negative di-mon mating, Esterase isoenzyme, RAPD, Sensitivity , Agronomic character , Homeonomous heterokaryon.
基金项目：本文由博士点基金项目与安徽省教育厅科研基金资助
作者单位：叶明（山东大学微生物技术国家重点实验室 济南 250100;安徽机电学院生物化工系 芜湖 241000）
潘迎捷（食用菌遗传育种农业部部级重点实验室 上海201106）
马桂荣（山东大学微生物技术国家重点实验室 济南 250100）
陈咏萱（南京农业大学微生物系 南京 210095）
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