食用菌遗传转化研究进展

食用菌遗传转化研究进展

Research Advances in Genetic Transformation of Edible Fungi

郭丽琼　陈守才　林俊芳　

摘　要：食用菌遗传转化的方法主要有PEG法、电激法、基因枪法、限制酶介导法、农杆菌介导法;采用的启动子有fas启动子和gpd启动子;采用的筛选标记有营养缺陷型标记、抗生素抗性筛选标记、除草剂和杀菌剂抗性筛选标记、代谢产物抗性筛选标记.本文综述了这些遗传转化方法以及启动子、筛选标记的最新研究进展.

关键词：食用菌;遗传转化;限制酶介导法;农杆菌介导法;启动子;筛选标记

分类号：S646.032　文献标识码：A

文章编号：1005-9873(2001)04-47-53

基金项目：国家自然科学基金资助项目(39960050)的一部分

作者简介：林俊芳责任作者

作者单位：郭丽琼（中国热带农业科学院热带作物生物技术国家重点实验室,海南海口571101）　

　　　　　陈守才（中国热带农业科学院热带作物生物技术国家重点实验室,海南海口571101）　

　　　　　林俊芳（中国热带农业科学院热带作物生物技术国家重点实验室,海南海口571101）　

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