双孢蘑菇性亲和性相关分子标记的初步筛选
Primary Screening of Molecular Markers Related to Sexual Compatibility in Agaricus bisporus
詹才新 凌霞芬 杨家林
摘 要：以传统的形态,生理生化分析和最新的DCS-PDMA性亲和性测定方法为基础,结合群体分离分析和RAPD技术对来源于同一双孢蘑菇异核体菌株的12个不育同核原生质体个体进行分析,筛选与性亲和性相关的分子标记.研究结果表明,供试的12个不育同核原生质体个体被分成两大类性亲和性类型,其中一类(A+)包括不育同核原生质体个体B、 C、 D、 E、 F、 G、 H、 I、 J、 L,另一类(A-)则仅仅包括不育同核原生质体个体K和M,同时筛选到一个与性亲和性相关的分子标记OPA161500.从而为间接地利用双孢蘑菇本身特有的交配型作标记来指导杂交育种工作和进一步将其性亲和性基因定位分离克隆奠定了坚实的基础.
关键词：双孢蘑菇; DCS-PDMA方法; 群体分离分析; 性亲和性相关分子标记; 杂交育种
分类号：S464.110.36 文献标识码：A
文章编号：1005-9873(2002)04-01-08
基金项目：国家自然科学基金项目(95-01-04)
作者简介：第一作者现在地址: Box 109, Laboratory of Developmental Neurobiology, the Rockefeller University,1230 York Avenue, New York, NY 10021, USA.杨家林，本文通讯作者
作者单位：詹才新（上海市农业科学院食用菌研究所;农业部食用菌遗传育种重点开放实验室,上海,201106）
凌霞芬（上海市农业科学院食用菌研究所）
杨家林（鄂州市卫生专科学校,鄂州,436000）
参考文献：
［1］杨新美. 中国食用菌栽培学[M]. 北京:农业出版社,1986. 247～250.
［2］Chang S. Mushroom research and development-equality and mutual benefit[C]. In: Rose DJ (ed) Mushroom biology and mushroom products. Proceedings of the Second International Conference, University Park, Pennsylvania, Penn State Univ., 1996. 1～10.
［3］Raper CA, Miller RE, Raper JR. Genetic analysis of the life cycle of Agaricus bisporus[J]. Mycologia, 1972, 64: 1088～1117.
［4］May B, Royse DJ. Confirmation of crosses between lines of Agaricus brunnescens by isozyme analysis[J]. Experimental Mycology, 1982, 6: 283～292.
［5］Castle AJ, Horgen PA, Anderson JB. Restriction fragment length polymorphisms in the mushroom Agaricus brunnescens and Agaricus bitorquis[J]. Appl Environ Microbiol, 1987, 53:816～822.
［6］马爱民,贺冬梅,潘迎捷. 双孢蘑菇同核原生质体育种技术研究[J]. 食用菌学报,1995,2(3]:1～5.
［7］Zhan Caixin. Determination of sexual compatibility by DCS-PDMA method in Agaricus bisporus[R]. China-Japan International Symposium on Mycology. Wuhan, China, 1997. 50～51.
［8］Zhan Caixin, Zhu Lanbao, Yang Xinmei. Distinguishing strains of Flammulina velutipes with RAPD[J]. Journal of Huazhong Agricultural University, 1995,14 (3): 253～258.
［9］Michelmore RW, Paran I, Kesseli RV. Identification of markers linked to resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genome regions by using segregating populations[J]. Proc Natl Acad Sci USA, 1991, 88: 9828～9832.
［10］Paran I, Michelmore RW. Development of reliable PCR-based markers linked to downy mildew resistance genes in lettuce[J]. Theor Appl Genet, 1993, 85: 985～993.
［11］Judelson HS, Spielman LJ, Shattock R. Mapping non-mendelian segregation of mating-type loci in the Oomycete, Phytophthora infestans[J]. Genetics, 1995,141:503～512.
［12］詹才新,张引芳,凌霞芬,等.中国主栽双孢蘑菇菌株的DNA多态性[J].食用菌学报,1998,5(2):1～7.
［13］詹才新,凌霞芬. 双孢蘑菇菌落形态和产质量性状间相关性研究[J].食用菌学报,1997,4(3):7～12.
［14］Kothe E. Mating-type genes for basidiomycete strain improvement in mushroom farming[J]. Appl Microbiol Biotechnol, 2001,56:602～612.
［15］Stoop JMH, Mooibroek H. Advances in genetic analysis and biotechnology of the cultivated button mushroom, Agaricus bisporus[J]. Appl Microbiol Biotechnol, 1999, 52: 474～483.
