王淑珍; 范俊; 扬家峰; 扬英杰; 白晨
上海师范大学生命与环境科学学院; 上海师范大学生命与环境科学学院; 上海师范大学生命与环境科学学院; 上海师范大学生命与环境科学学院; 复旦大学生命科学学院 上海200234 ; 上海200234 ; 上海200234 ; 上海200234 ; 上海200433
【中文摘要】 以获得弱木栓质化灵芝菌株为目的 ,利用液体培养 4d的灵芝菌丝体和液体培养 3d的糙皮侧耳菌丝体为材料 ,在PEG Ca2 + 系统中诱导原生质体融合 ,获得初筛融合子 19株 (F1～F19)。其中 ,融合子F8的菌丝生长速度和生物量均接近糙皮侧耳 ,显微镜下 ,其菌丝体与灵芝和糙皮侧耳的均不相同 ,木栓质化程度大于糙皮侧耳 ,小于灵芝。
【英文摘要】 In order to obtain the lower corkification strain of Ganoderma lucidum, the mycelia of G.lucidum and Pleurotus ostreatus were put under submerged culture for 4d and 3d resp.to prepare the protoplasts. And the fusion experiment of G.lucidum and P.ostreatus protoplasts was carried out under PEG-Ca + system. Ninteen fusants numbered from F1 to F19 were selected. Among them, the mycelial growth rate and the mycelial biomass of F8 were more similar to that of P.ostreatus, and the mycelia of F8 were very different from that of G.lucidum and P.ostreatus under the microscope.Furthermore, the corkification level of F8 mycelia was between that of G.lucidum and P.ostreatus .
【中文关键词】 灵芝; 糙皮侧耳; 融合子; 生物学特性
【英文关键词】 Ganoderma lucidum; Pleurotus ostreatus; Fusant; Biological characteristic
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2003年02期 【DOI】CNKI:SUN:SYJB.0.2003-02-001