王慧杰; 李宇伟; 连瑞丽; 王志强; 马爱民
郑州牧业工程高等专科学校生物工程系; 郑州牧业工程高等专科学校生物工程系; 郑州牧业工程高等专科学校生物工程系; 上海农业科学院食用菌研究所; 华中农业大学食品科技学院 河南郑州450011; 河南郑州450011; 河南郑州450011; 上海201106; 湖北武汉430070
【中文摘要】 从一系列灵芝(Ganoderma sp.)菌种中筛选出多糖产量高、生长快的紫灵芝菌株(G.japoncium),研究了不同氮源、碳源及金属离子对紫灵芝产多糖的影响。结果表明,以2%的蔗糖为碳源,0.2%的豆饼粉为氮源,0.2%的FeSO4为培养基可获得较高多糖。发酵罐放大实验表明,采用同样的培养基,每100mL发酵液胞外粗多糖含量可高达181.7mg,每100mL发酵液菌丝体含量可高达151.0mg,发酵过程中pH值的变化比较缓和,相对摇瓶生长,发酵生产可获得更多的灵芝多糖。
【英文摘要】 A strain of Ganoderma japonicum was selected from seven Ganoderma test strains based on a higher mycelial growth rate and ability to produce higher levels of polysaccharide. Comparison of selected nutritional parameters affecting polysaccharide production in small-scale submerged flask cultures revealed that a fermentation medium containing 2% sucrose as carbon source, 0.2% bean cake as nitrogen source, and supplemented with 0.2% FeSO_4 yielded peak amounts of 181.7 mg crude polysaccharide and 151.0 mg fungal biomass per 100 mL fermentation liquid. However, compared with the flask cultures, higher yields of G. japonicum polysaccharide were obtained by submerged culture in a fermenter.
【中文关键词】 提取; 发酵; 灵芝; 条件优化; 多糖
【英文关键词】 Extraction; Fermentation; Ganoderma spp.; Optimization of nutritional parameters; Polysaccharide
【基金】河南省科技攻关项目(编号:0124050032)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2007年01期 【DOI】CNKI:SUN:SYJB.0.2007-01-009