【作者】郭佳; 宋荣; 包海鹰; 崔宝凯
【Author】GUO Jia1; SONG Rong1; BAO Haiying1; CUI Baokai21College of Traditional Medicine; Jilin Agricultural University; Changchun; Jilin 130118; China; 2Institute of Microbiology; Beijing Forestry University; Beijing 100083; China
【作者机构】吉林农业大学中药材学院; 北京林业大学微生物研究所
【摘要】 选用H22荷瘤小鼠对火木层孔菌(Phellinus igniarius)和瓦尼木层孔菌(Phellinus vaninii)发酵产物进行抗肿瘤体内试验,测定其对H22荷瘤小鼠抑瘤率、免疫器官指数、免疫因子含量和生存率的影响。结果表明,火木层孔菌和瓦尼木层孔菌发酵产物高、低剂量组(1 000 mg/kg、500 mg/kg)均对肿瘤具有一定抑制作用,其中瓦尼木层孔菌发酵产物低剂量组(500 mg/kg)抑瘤效果最佳,其抑瘤率为58.7%。火木层孔菌和瓦尼木层孔菌发酵产物高、低剂量组均可提高小鼠血清中白细胞介素2(interleukin-2,IL-2)的含量、延长小鼠的生存期,但是对血清中肿瘤坏死因子(tumor necrosis factor-,αTNF-α)含量的影响作用不显著。
【Abstract】 Whole cultures(mycelium and spent broth) of Phellinus igniarius and Phellinus vaninii grown for 8 days at 30 ℃ on a medium containing sucrose,peptone and mineral salts,were evaporated to dryness in an oven for 24 h at 35 ℃.After grinding to a powder,the solid residues were suspended in physiological saline and the anti-tumor activity of the two preparations(FPPI-P.igniarius,and FPPV-P.vaninii) was determined using H22 tumor-bearing mice as the test model.Both low(500 mg/kg/d) and high(1 000 mg/kg/d) doses of FPPI and FPPV inhibited tumor growth.The inhibitory effect was most pronounced(58.7%) with low doses of FPPV,which was virtually identical to that recorded in the 5-fluorouracil(5-Fu) positive control group.Both low and high doses of FPPI and FPPV also increased interleukin-2(IL-2) levels in test mice and prolonged their survival time.
【关键词】 火木层孔菌; 瓦尼木层孔菌; 抗肿瘤
【Key words】 Phellinus igniarius; Phellinus vaninii; antitumor activity
【基金】国家自然科学基金项目“药用木腐真菌‘桑黄’资源及其抗肿瘤活性的研究”(编号:30870013)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2010年02期
【DOI】CNKI:SUN:SYJB.0.2010-02-017
【分类号】S567.39