【作者】王钦博; 杨焱; 刘艳芳; 高君辉; 张劲松; 唐庆九; 冯娜; 周帅
【Author】WANG Qinbo1; 2; YANG Yan1; LIU Yanfang1; GAO Junhui1; ZHANG Jinsong1; TANG Qingjiu1; FENG Na1; ZHOU Shuai11National Engineering Research Center for Edible Fungi Biotechnology and Engineering; Key Laboratory of Applied Mycological Resources and Utilization; Ministry of Agriculture; Key Laboratoryof Agricultural Genetics and Breeding of Shanghai; Research Center of Natural Medicinal Resources; Instituteof Edible Fungi; Shanghai Academy of Agricultural Sciences; Shanghai 201106; China; 2College of Life and Environmental Sciences; Shanghai Normal University; Shanghai 200234
【作者机构】国家食用菌工程技术研究中心农业部应用真菌资源与利用重点开放实验室上海市农业遗传育种重点开放实验室上海市农业科学院天然药物研究与开发中心上海市农业科学院食用菌研究所; 上海师范大学生命与环境科学学院
【摘要】 测定了一年生、两年生桑黄(Phellinus baumii)子实体水提物中的粗多糖、醇提物中的黄酮和三萜含量,并研究了不同生长年份子实体水提粗多糖和醇提物体外刺激淋巴细胞生长、抑制L1210肿瘤细胞生长的作用及抗氧化活性。结果表明,一年生桑黄子实体醇提物中黄酮、三萜和水提物中粗多糖的含量略高于两年生桑黄;当水提粗多糖浓度为10μg/mL时,SH2-W对体外淋巴细胞的增殖率高于SH1-W,当浓度为50和200μg/mL时,两组样品间无显著差异;两种桑黄醇提物对体外肿瘤细胞L1210的抑制作用随浓度增大而增强,相同剂量下,两组样品间无差异;相同浓度下SH1-E对H2O2的清除率高于SH2-E,但两组样品DPPH.的清除率和还原力无显著差异。
【Abstract】 One-and two-year-old Phellinus baumii fruit bodies were extracted with water(SH1-W and SH2-W,respectively) or ethanol(SH1-E and SH2-E,respectively),and the polysaccharide content of the aqueous extracts,and the flavone and triterpene content of the ethanolic extracts,were determined.Polysaccharide levels in fraction SH1-W,and flavone and triterpene levels in fraction SH1-E,were higher than in the corresponding extracts of two-year-old sporophores.At 10 μg/mL concentration,fraction SH2-W was more effective than fraction SH1-W at stimulating lymphocyte proliferation,but there was no significant difference in the stimulating effects of the two extracts at higher concentrations(50 and 200 μg/mL).Inhibition of L1210 tumor cell growth in vitro by fractions SH1-E and SH2-E increased with increasing extract concentrations but there was little difference in the inhibitory effects of the two extracts at the same concentration level.The H2O2 scavenging capacity of SH1-E was higher compared with S...更多H2-E at the same concentration,but the two extracts exhibited similar DPPH· scavenging activity and reducing power. 还原
【关键词】 桑黄子实体; 粗多糖; 黄酮; 抗氧化; 淋巴细胞增殖
【Key words】 Phellinus baumii; fruit body; polysaccharide; flavonoid; antioxidant activity; lymphocyte proliferation
【基金】桑黄黄酮生物制品制备技术中试优化(编号:093919N0900)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2010年02期
【DOI】CNKI:SUN:SYJB.0.2010-02-015
【分类号】S567.39