【作者】刘春滟; 李南羿; 张玉琼
【Author】LIU Chunyan1; LI Nanyi2; ZHANG Yuqiong11College of Life Science; Anhui Agricultural University; Hefei; Anhui 230036; China; 2 College of Agriculture and Food; Zhejiang Forestry University; Lin’an; Zhejiang 311300; China
【作者机构】安徽农业大学生命科学院; 浙江林学院农业与食品学院
【摘要】 从NCBI中下载了12 184条香菇(Lentinula edodes)表达序列标签(expressed sequence tag,EST)序列,处理后得到全长3 681 177 bp的4 684个Unigene。在其中共发掘出142个简单重复序列(simple sequencerepeat,SSR),分布于120条EST中,分布频率为2.99%(平均分布距离:25.924 kb)。其中,单、二、三核苷酸重复是主要的重复类型,A/T,AG/CT,ACG/CTG是单、二、三核苷酸的主要重复基序,分别占所有EST-SSR的23.23%、21.83%和10.56%。利用引物设计软件PrimerPremier5.0设计了40对引物,并利用6%变性聚丙烯酰胺凝胶在18个香菇品种中进行检测。其中,22对引物为多态性引物,多态率为55%。应用NTSYS软件的聚类方法分析表明,18份材料遗传相似系数范围为0.3750.984,与前人研究结果相符。
【Abstract】 A total of 12 184 Lentinula edodes expressed sequence tags(ESTs) were downloaded from NCBI and shown to be associated with 4 684 Unigenes comprising 3 681 177 bp in length.A MISA search revealed a total of 142 simple sequence repeats(SSRs) with a frequency of 2.99% and an average distance distribution of 25.9 kb.The most abundant mononucleotide,dinuclotide and trinucleotide repeats were A/T,AG/CT and ACG/CTG,accounting for 23.23%,21.83% and 10.56% of each type,respectively.Forty EST-SSR primer pairs were designed by PrimerPremier 5.0,22 of which revealed polymorphic bands in the DNA of 18 L.edodes strains.NTSYS-computed similarity coefficients for the 18 strains ranged between 0.3750.984,which was approximately in accordance with their diversity based on previous research.
【关键词】 香菇; EST序列; SSR标记; 多态性; 遗传多样性
【Key words】 Lentinula edodes; expressed sequence tag(EST); simple sequence repeat(SSR) marker; polymorphism; genetic diversity
【基金】上海市农业遗传育种重点实验室开放课题“香菇EST-SSR标记的开发、鉴定及其在种质资源多样性分析中的应用”(编号:shagb2009-03)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2010年02期
【DOI】CNKI:SUN:SYJB.0.2010-02-001
【分类号】S646.12