钱秀萍; 杨庆尧
上海师范大学生物系; 上海师范大学生物系 上海 200234 ; 上海 200234
【中文摘要】 裂褶菌在发酵培养基中振荡培养产生苹果酸的最佳碳源是葡萄糖,每毫升发酵液产苹果酸10.48mg,果糖和麦芽糖次之,苹果酸产量分别为6.28mg/ml、5.05mg/m1.最佳氮源是牛肉浸膏、草酸铵和硝酸铵,苹果酸产量分别为6.65mg/ml。6.05mg/ml、5.98mg/ml,苹果酸产率分别是16.5%、13.3%、12.5%.在自来水配制的发酵培养基中,磷酸二氢钾和氯化钾对裂褶菌产酸影响不大,而培养基中添加硫酸镁后苹果酸产量下降。碳酸钙浓度与苹果酸产量成正比,碳酸钙浓度从1%增加到5%,苹果酸产量从1.55mg/ml提高到11.38mg/ml,产率可提高约7倍。
【英文摘要】 L-malic acid could be produced by Schizophyllum commune through shake-culture in fermentative medium. It was proved that the best carbon source was glucose producing 10.48mg/ml malic acid in the fermentative medium, followed by fructose and maltose with a malic acid yield of 6.28mg/ml and 5.05 mg/ml resp.. The most favourable nitrogen sources were proved to be beef extract,ammonium oxalate and ammonium nitrate. Their malic acid yields were .6.65mg/ml, 6.05mg/ml and 5.98mg/ml resp. with the productive rate being 16.5%, 13.3% and 12.5% resp.. It was found that KH2PO4 and KC1 hardly affected Schizophyllum commune to produce acid in fermentative medium made up with tap water , but MgSO4 decreased the yield of malic acid. When the concentration of CaCO3 was raised from 1% to 5%, the malic acid yield was increased from 1.55mg/ml to 11.38mg/ml and the productive rate was increased by a factor of about 7.
【中文关键词】 裂褶菌; 发酵; 苹果酸; 碳源; 氮源; 无机盐
【英文关键词】 Schizophyllum commune; Fermentation; Malic acid; Carbon source; Nitrogen source; Inorganic salt
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,1996年03期 【DOI】CNKI:SUN:SYJB.0.1996-03-003
