宋爱荣; 张玉娜; 孙克梅; 张英昊; 万平平
莱阳农学院植保系; 莱阳农学院植保系; 莱阳农学院植保系; 莱阳农学院植保系; 莱阳农学院植保系 山东莱阳265200 ; 山东莱阳265200 ; 山东莱阳265200 ; 山东莱阳265200 ; 山东莱阳265200
【中文摘要】 在液体培养条件下测定了发酵液中 5个灰树花菌株蛋白质含量的变化。结果表明 ,接种后第 6天发酵液中蛋白质含量最高 ,Gf96 1菌株发酵液中的蛋白质含量为 0 .5 8% ,Gf96 2菌株发酵液中的蛋白质含量最低 ,为 0 .30 %。随着培养时间的延长 ,发酵液中蛋白质含量逐渐下降。接种后第 9天最低 ,随后又呈上升趋势 ,但上升的幅度较小。试验为确定从发酵液中提取蛋白质的最佳时期提供了一定的理论依据
【英文摘要】 The variation of protein content of 5 Grifola frondosa strains under submerged culture conditions was determined in this study. The result showed that the protein content of the fermented broth was the highest at the sixth day of spawning, and that the protein content of Gf961 in the fermented broth was the highest(0.58%), while that of Gf962 was the lowest(0.30%). It was also confirmed in our experiments that the protein content in the fermented broth reduced gradually along with the extending of the culture time, and reached the lowest at the 9th day of spawning, but it increased again later with small rate of increment. So the experiment result offered some theoretical bases for the determination of optimum time to extract proteins from the fermented broth.
【中文关键词】 灰树花; 发酵液; 蛋白质; 深层培养
【英文关键词】 Grifola frondosa; Fermented broth; Protein; Submerged culture
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2002年03期 【DOI】CNKI:SUN:SYJB.0.2002-03-002
