李翠翠; 郭立忠; 卢伟东; 董伟
青岛农业大学山东省应用真菌省级重点实验室
【中文摘要】 利用RAPD及SRAP分子标记技术对从国内外收集到的12个真姬菇菌株进行遗传多样性分析。结果表明,20条RAPD引物共扩增出多态性条带285条,18对SRAP引物共扩增出多态性条带176条。在相似系数0.800水平时,RAPD和SRAP分子标记分别将12个真姬菇菌株分为5个和6个类群。两种方法均适用于真姬菇种内鉴定,而SRAP标记较RAPD标记稳定性好,更适于真姬菇的种内鉴定。
【英文摘要】 Randomly amplified polymorphic DNA(RAPD)and sequence-related amplified polymorphisms(SRAP)were used to identify 12 strains of Hypsizygus marmoreus Sing.A total of 285 and 176 polymorphic bands were amplified by 20 RAPD primers and 18 SRAP primer pairs,respectively.Two dendrograms based on UPGMA(unweighted pair group method with arithmetic average)cluster analysis using the amplification data revealed that,at the similarity level of 0.8,the 12 strains clustered into five and six groups,respectively.We conclude that both methods are suitable for discriminating among strains of H.marmoreus,and that the SRAP markers were more effective in reflecting genetic diversity.
【中文关键词】 真姬菇; RAPD; SRAP; 聚类分析
【英文关键词】 Hypsizygus marmoreus; RAPD; SRAP; clustering analysis
【基金】国家科技支撑计划子课题“秸秆栽培食用菌高产高效技术模式与示范”(编号:2007BAD89B09-15);; 公益性行业科研专项子课题“非热灭菌秸秆食用菌培养基关键技术研究”(编号:200803033-B0604)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2009年01期 【DOI】CNKI:SUN:SYJB.0.2009-01-005
