张红玉; 付立忠; 吴学谦; 李海波; 魏海龙; 吴庆其; 王立安
河北师范大学生命科学学院; 浙江省林业科学研究院; 浙江省森林资源生物与化学利用重点实验室; 浙江省林业科学研究院浙江省森林资源生物与化学利用重点实验室; 丽水市食用菌研究开发中心; 浙江益圣菌物发展有限公司
【中文摘要】 为验证新型SRAP分子标记技术用于灵芝(Ganoderma lucidum)原生质体融合子鉴定的可行性,通过亲本菌株赤芝05、06对153个SRAP引物组合进行筛选,应用经过初筛和复筛得到的4个清晰稳定、且含有两个亲本各自特异扩增条带的SRAP引物组合,对2个亲本菌株、2个只与亲本05有拮抗的融合子、2个只与亲本06有拮抗的融合子、30个与双亲均有拮抗的融合子进行SRAP扩增。结果表明,2个只与亲本05有拮抗的融合子仅扩增出06的特异条带,2个只与亲本06有拮抗的融合子仅扩增出05的特异条带,30个与双亲均有拮抗的融合子中,有7个融合子同时扩增出05和06的特异条带,证明此7个融合子应是05和06融合产生的菌株,也证明SRAP标记可以用于灵芝原生质体融合子的鉴定。
【英文摘要】 SRAP makers were used to identify protoplast fusion hybrids of Ganoderma lucidum.A total of 153 SRAP primer combinations were selected using G.lucidum parental strains 05 and 06.Four primer pairs generated clear,stable and specific bands for each of the parental strains,and were used to confirm protoplast fusion hybrids.A total of 181 strains were isolated from regenerated protoplasts after fusion treatment of equal numbers of protoplasts prepared from each of the two parental strains.These strains were divided into three groups based on their antagonistic effect with the two parental strains: 35 isolates exhibited antagonism with parental strain 05(Group A),116 isolates with parental strain 06(Group B),and 30 isolates with both parental strains(Group C).SRAP amplification was then carried out with the two parental strains,with two isolates each from A and B groups,and with all the Group C isolates using the four selected SRAP primer pairs.Bands specific only for parental strain 06 wer...更多e observed with Group A isolates,whereas Group B isolates generated bands specific only for parental strain 05.However,bands specific for both parental strains were observed with seven Group C isolates,thereby confirming these isolates to be true protoplast fusion hybrids. 还原
【中文关键词】 药用菌; 灵芝; SRAP分子标记; 原生质体融合; 杂种鉴定
【英文关键词】 Medicinal mushrooms; Ganoderma lucidum; SRAP molecular marker; protoplast fusion; hybrid identification
【基金】浙江省科研农业项目“食用菌杂交育种亲本选择及杂交子分子鉴定技术研究”(编号:2007C22069);; 浙江省青年人才培养项目“食药用真菌种质资源遗传评价及DNA分子标记鉴定技术研究”(编号:2006R20006);; 浙江省创新团队建设与人才培养项目“现代食用菌科技创新团队建设”;; 浙江省森林资源生物与化学利用重点实验室项目“高多糖、高三萜含量香菇、灵芝种质创制与菌株筛选”的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2009年04期 【DOI】CNKI:SUN:SYJB.0.2009-04-001
