【作者】汪虹; 陈明杰; 冯爱萍; 乔娜
【Author】WANG Hong; CHEN Mingjie; FENG Aiping; QIAO Na National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization; Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; 201106; P. R. China
【作者机构】国家食用菌工程技术研究中心; 农业部应用真菌资源与利用重点开放实验室; 上海市农业遗传育种重点开放实验室; 上海市农业科学院食用菌研究所
【摘要】 将草菇(Volvariella volvacea)菌株V23和耐低温诱变菌株VH3的菌丝体在4℃下处理不同时间后,采用荧光定量PCR技术中的双标准曲线法研究了S-腺苷-L-高半胱氨酸水解酶同源基因Cor3的表达变化。结果显示,低温处理后,草菇V23菌株的Cor3基因表达量随处理时间的延长呈不断下降趋势,10h时,其表达量仅为对照的7%。而VH3菌株Cor3基因4℃处理2h表达量明显上升,为对照的1.8倍,处理4h和6h表达量降低,分别为对照的80%和90%,处理8h的表达量又上升,为对照的1.4倍,随后下降,处理10h表达量最低,为对照的60%。根据结果初步推测Cor3基因与草菇低温耐受性具有一定相关性。
【Abstract】 The high temperatures necessary for vegetative growth and fruiting of the edible mushroom,Volvariella volvacea,restrict industrial scale cultivation to tropical and sub-tropical regions,thereby limiting the wider industrial exploitation of this important edible species. As part of a study aimed at developing commercially viable,cold-tolerant strains of V. volvacea capable of growing and fruiting in temperate climes,we have used real-time quantitative PCR to compare expression levels of a gene encoding for S-adenosyl-L-homocysteine hydrolase (Cor3) in two strains of V. volvacea,a low-temperature sensitive strain (V23) requiring temperatures >28 ℃ for normal vegetative growth and fruiting,and a cold-tolerant mutant (VH3),following exposure to 4 ℃. Relative expression levels of Cor3 in parental V23 mycelia exhibited a general decrease with time following exposure,declining by 80% and 30% of initial values after 2 h and 4 h,respectively. After 10 h,the transcription level was only 7% o...更多f that prior to low temperature exposure. Conversely,relative Cor3 expression levels in mutant VH3 mycelia exposed to 4 ℃ were proportionately higher throughout the treatment period. Expression levels after 2 h were 1.8-fold higher than initial levels,and then fluctuated between 80%-90% of zero time levels after 46 h. Gene transcription again increased (to 140% of zero time levels) after 8 h,and then fell to 60% of initial levels after 10 h exposure. Our data indicate that low levels of Cor3 expression are linked to the low temperature sensitivity of V. volvacea mycelium. 还原
【关键词】 冷诱导基因表达; 低温耐受性; 实时定量PCR; S-腺苷-L-高半胱氨酸水解酶; 草菇
【Key words】 Cold-induced gene transcription; cold tolerance; real-time quantitative PCR; S-adenosyl-L-homocysteine hydrolase; Volvariella volvacea
【基金】上海农科院2008年度院发基金[编号:上海农科院农科发2008(16)];; 上海科技兴农重点攻关项目(编号:沪农科攻字2007第6-2号);; 农业部公益性行业科研专项项目(编号:nyhyzx07-008)的部分研究内容~~
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2010年01期
【DOI】CNKI:SUN:SYJB.0.2010-01-005
【分类号】S646.13
