唐庆九; 张劲松; 潘迎捷; Werner Reutter; 樊华
Institut für Molekularbiologie und Biochemie; Institut für Molekularbiologie und Biochemie; 上海市农业科学院食用菌研究所; Institut für Molekularbiologie und Biochemie; Institut für Molekularbiologie und Biochemie Freie Univertt; Berlin; Germany; 上海市农业科学院食用菌研究所; 上海201106 ; Freie Univertt; Berlin; Germany; 上海市农业科学院食用菌研究所; 上海201106 ; 上海201106 ; Freie Univertt; Berlin; Germany ; Freie Univertt; Berlin; Germany
【中文摘要】 运用高通量筛选技术检测了几种药用真菌粗提物对人体肿瘤细胞增殖的抑制作用和对小鼠巨噬细胞的激活作用,并与粗提物透析袋外的小分子成分的活性进行比较。结果显示,灵芝、猴头、云芝和灰树花粗提物对人肠腺癌细胞株SW620有很明显的抑制作用,并有显著激活小鼠巨噬细胞的作用。粗提物经过透析后,透析袋外的小分子成分对SW620的抑制作用与粗提物相似,但对小鼠巨噬细胞没有明显的激活作用。本研究所采用的高通量筛选技术为进一步筛选药用真菌的有效成分奠定了基础。
【英文摘要】 The proliferationinhibition for tumour cells and the activation of mouse macrophages by the crude extracts of Ganoderma lucidum, Grifola frondosa, Hericium erinaceus and Coriolus versicolor were investigated using highthroughput screening technique, and the activity was also compared with that of their small molecular part outside the dialytic bag of the crude extracts. The results showed that the crude extracts of above mentioned four medicinal fungi inhibited the proliferation of human bowel glandula tumour cells of strain SW620 and activated the mouse macrophages significantly. And the growth of SW620 cells were also inhibited significantly by the small molecular part dialyzed from the crude extracts, but the activation of the mouse macrophages was not stimulated remarkably by these small molecular parts. It was expected that the highthroughput screening technique used in the research provided the basis for further screening the active components from the medicinal fungi.
【中文关键词】 高通量筛选技术; 云芝; 灵芝; 猴头; 灰树花
【英文关键词】 High-throughput screening technique; Coriolus versicolor; Ganoderma lucidum; Hericium erinaceus; Grifola frondosa
【基金】德国"BundesministriumfürForschungundTechnologie,Bonn."科研基金资助项目(编号SFB366)
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2003年03期 【DOI】CNKI:SUN:SYJB.0.2003-03-001