罗信昌
华中农业大学 武汉
【中文摘要】 本文报导使用Novozym234酶从光木耳(Auricularia auricula)和毛木耳(Auricularia polytricha)单核菌丝体中,成功地分离出高产量的原生质体;一些因素对原生质体形成的影响及原生质体再生过程中形态学变化的观察结果。菌体的液体培养时间、缓冲液pH值和一定浓度的酶液用量与原生质体的形成有着密切的关系。原生质体的两种再生型能在液体的CYM(Complete yeast extract medium)和固体的CYM含有0.6M山梨醇的再生培养基中观察到。
【英文摘要】 The formation and regeneration of mycelial protoplasts from Auricularia auricula and Auricularia polytricha using Novozym 234 were examined. The amour of release of protoplasts is related to mycelial age, buffet pH and dose of enzyme used. In general, liquid culture for 10 days or buffer pH 5—5.9 and the 3ml 2% of Novozym 234 per gram wet weight mycelia are suitable for protoplast formation in both strains. The regeneration frequency of protoplasts of both strains was found by using RM medium, 3.4% for Auricularia polytricha and 3.2% for Auricularia auricula. Tworvegeneration types were observed: Protoplasts directly produced one or more germ tubes and developed into bud-like structures of cells before producing germ tubes by using LRM medium.
【中文关键词】 原生质体; 单核体; 再生型; 光木耳; 毛木耳
【英文关键词】 protoplast; Monocaryon; Regeneration type; Auricularia auricula; A. polytricha.
【基金】中国科学院基金
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,1987年01期 【DOI】CNKI:SUN:ZSYJ.0.1987-01-000
