陈瑜; 梁运祥; 胡咏梅; 葛向阳
湖北华中农业大学农业微生物学国家重点实验室; 湖北华中农业大学农业微生物学国家重点实验室; 湖北华中农业大学农业微生物学国家重点实验室; 湖北华中农业大学农业微生物学国家重点实验室 武汉430070; 武汉430070; 武汉430070; 武汉430070
【中文摘要】 以苯酚硫酸法作为灵芝多糖的测定方法,研究了从固体发酵灵芝菌丝体中提取水溶性多糖的工艺,对浸提过程中的温度、时间、固液比及提取次数4个因素进行了单因素试验和正交试验,并利用SephadexG-100凝胶色谱法和薄层层析法对主要组分GLPⅠ进行了分子量测定和单糖分析。结果表明,灵芝多糖的最佳提取条件为浸提温度95℃,浸提时间40min,固液比1∶20和提取次数为2次;GLPⅠ分子量约为179900,初步判断为甘露聚糖。
【英文摘要】 Using phenol-sulphuric acid method to detect the contents of polysaccharides, the extraction of the water-soluble polysaccharides from Ganoderma lucidum mycelia was studied. The optimum extraction conditions were determined by single-factor test and orthogonal test. And the molecular weight and monosaccharide composition of GLPⅠ were determined by Sephadex G-100 gel filtration chromatography and thin-layer chromatography. The results showed that the optimum extracting temperature, time, ratio of mycelia to water ( w∶v ) and extracting times were 95℃, 40 min, 1∶20 and 2 times. The molecular weight of GLPⅠ was approximately 179 900. GLP I was probably composed of D-mannose.
【中文关键词】 单因素试验; 正交试验; 凝胶色谱; 薄层层析法
【英文关键词】 Single-factor test; Orthogonal test; Gel filtration chromatography; Thin-layer chromatography
【基金】浙江省重大科技攻关项目(No.2004C1210)
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2007年03期 【DOI】CNKI:SUN:ZSYJ.0.2007-03-015
