马金宝; 沈业寿; 李峰; 史新强; 赵根海; 解要敏
安徽大学生命科学学院生化微生物研究所安徽省生态工程与生物技术重点实验室
【中文摘要】 采用邻苯三酚自氧化法、Fenton反应体系法、DPPH体系法,以抗坏血酸为阳性对照,对亮菌多糖-1b(ATPS-1b)清除超氧阴离子自由基(O2-·)、羟自由基(·OH)和二苯代苦味肼基自由基(DPPH·)的效果进行了测定。结果表明ATPS-1b对O2-·、·OH和DPPH·3种自由基均具有很强的清除作用,且存在良好的量效关系,随着ATPS-1b浓度的增大,其清除效果增强;清除O2-·、·OH、DPPH·3种自由基的EC50值分别为0.69mg·mL-1、8.73mg·mL-1、2.01mg·mL-1;对3种自由基的最大清除率分别为86.17%、76.4%、78.52%;ATPS-1b具有显著的体外抗氧化活性。
【英文摘要】 The effects of polysaccharide-1b from Armillariella tabescens(ATPS-1b) to remove superoxide anion free radical,hydroxyl free radical and DPPH· free radical were determined by the methods of the auto-oxidation of pyrogallol system,Fenton reaction system and DPPH· system. The results showed that ATPS-1b had strong scavenging capacity to O2-·,·OH and DPPH·. Furthermore,scavenging capacity and anthocyanins concentration showed a position of dose-response relationship. The three free radicals had strong scavenging results with the increasing of concenteation. The EC50 of scavenging free radicals were 0.69 mg·mL-1,8.73 mg·mL-1,2.01 mg·mL-1respectively and maximum scavenging rates were 86.17%,76.4%,78.52%. ATPS-1b had significant antioxidant activity in vitro experiments.
【中文关键词】 亮菌; 多糖; 超氧阴离子自由基; 羟自由基; 二苯代苦味肼基自由基; 抗氧化活性
【英文关键词】 Armillariella tabescens; Polysaccharide; Superoxide anion free radical; Hydroxyl free radical; DPPH· free radical; Antioxidant activity
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2008年06期 【DOI】CNKI:SUN:ZSYJ.0.2008-06-018
