刘映淼; 王兰青; 高玉千; 戚元成; 申进文; 邱立友
河南农业大学生命科学学院
【中文摘要】 食用菌液体菌种由于具有不耐贮藏和不便于运输等缺点,极大地限制了液体菌种的推广应用。研究报道了一种食用菌液体菌种固化技术,可有效解决液体菌种的上述缺点。在优选的蔗糖、豆饼粉液体培养基中摇瓶培养香菇856,将得到的菌丝球与优选的主要成分是棉籽壳的固化载体基质按1∶6的比例混合,制成固化菌种,室温保藏30d,与保藏0d的固化菌种相比,呼吸强度低,纤维素酶活力明显升高,萌发力强,菌丝生长快。
【英文摘要】 The application and popularization of edible mushroom liquid spawn had been limited greatly due to its short storage time and shiping inconvenience. The liquid spawn solidification technique discussed in the present report would be a facility resolution to overcome the disadvantages of liquid spawn. Lentinus edodes 856 was cultivated by shaking flask in a selected optimum liquid medium, in which sugar and soybean cake powder were main compositions. Solidification spawn was made of the mixture of hyphae pellet and optimum solid carrier medium composed of cottonseed hull mainly, and the proper proportion between hyphae pellet and carrier medium was 1∶6. Compared to that of the solidification spawn stored for 0 d, the breathe intensity of solidification spawn stored for 30 d was decreased, and cellulase activity was otherwise higher and growth faster.
【中文关键词】 香菇; 液体菌种; 固化技术
【英文关键词】 Lentinus edodes; Liquid spawn; Solidification technique
【基金】河南省科技攻关项目(编号:0524040003)。
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2009年01期 【DOI】CNKI:SUN:ZSYJ.0.2009-01-009
