董昌金; 江涓; 姚占芳
湖北师范学院生物系!黄石435002; 湖北师范学院生物系!黄石435002; 河南农业大学生物工程学院!郑州450002
【中文摘要】 利用RAPD和酯酶同工酶技术研究了 6个香菇品种间的遗传差异和亲缘关系。通过聚类分析发现 ,类间组合杂种优势强 ,类内组合杂种优势弱 ,因此 ,杂交亲本应选择不同类的菌株。通过单孢杂交和出菇试验发现 ,杂交子代酶谱出现“互补型酶带”和“杂种新酶带” ,杂交后代杂种优势强 ,容易选出优良品种
【英文摘要】 The genetic variance and genetic relationship of six Lentinula edodes strains were studied with RAPD and esterase isoenzyme techniques. The result showed that the heterosis was stronger when the cross combinations were produced by parent strains in different groups,while the heterosis was weaker when the cross combinations were produced by parent strains in the same group. So parent strains in different groups should be selected for the crossbreeding. When mutually complementary enzyme bands and new enzyme bands of the new cross combinations appeared in the zymogram of the crossbreeding filial generation through monospore crossbreeding and fruiting experiment, the heterosis of the crossbreeding filial generation was stronger and high quality strains could be selected easily.
【中文关键词】 香菇; RAPD; 酯酶同工酶; 单孢杂交; 育种; 杂种优势
【英文关键词】 Lentinula edodes; RAPD; Esterase isoenzyme; Monospore crossbreeding; Heterosis
【基金】河南省科委攻关项目
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2000年01期 【DOI】CNKI:SUN:SYJB.0.2000-01-000
