罗联忠; 林树钱; 谢宝贵; 林志彬
福州绿谷生物药业技术研究所; 福州绿谷生物药业技术研究所; 福建农林大学菌物研究中心; 福州绿谷生物药业技术研究所 福州350002; 福建农林大学菌物研究中心; 福州350002 ; 福州350002 ; 福州350002 ; 福州350002; 北京大学医学部药理系; 北京100083
【中文摘要】 CTAB法提取灵芝菌丝体总DNA,再以核糖体DNA转录间隔区(ITSⅡ、IGRⅠ-PCR RFLP)结合随机引物扩增多态性(RAPD)标记分析30个灵芝菌株间的亲缘关系。结果表明,ITSⅡI、GRⅠ-PCR RFLP产生了47条带,其中42条显示出多态性,通过聚类分析将30个菌株分成七大类,包括紫芝、黑灵芝、树舌灵芝、薄树灵芝四大类,以及另外三个无法确定具体归属的类群,但没能将赤芝与松杉灵芝区分开。RAPD从247个随机引物中筛选出8个随机引物,共检测到69个多态性位点并全部显示出多态性,通过聚类分析将供试的30个灵芝菌株全部区分开。
【英文摘要】 Total DNA of Ganoderma mycelium was extracted by the method of CTAB,and genetic relationships among thirty Ganoderma strains were analyzed based on ITS Ⅱ、IGR Ⅰ-PCR RFLP and RAPD.The results showedthat 47 bands were obtained based on ITSⅡ,IGR Ⅰ-PCR RFLP,and 42 bands of them were polymorphic.Thirty strains were divided into seven species: Ganoderma sinense,Ganoderma atrum,Ganoderma applanatum,Ganaderma capense(Loyd) Teng and other three groups(their species are still unknown) through dendrogram aralysis.Only 8 primers produced stable results among 247 random primers by RAPD.Total 69 loci were obtained by using 8 primers and all of them were polymorphic. Based on these 69 loci,thirty Ganoderma strains were differentiated.
【中文关键词】 灵芝; 核糖体DNA转录间隔区; 随机引物扩增多态性
【英文关键词】 Ganoderma; Internal transcribed spacer(ITS); Rondom amplified polymorphic DNA(RAPD)
【基金】绿谷集团有限公司灵芝专项科研资金全额资助项目“灵芝菌种DNA鉴别”(项目编号:2003SHGV01);; 福建省星火计划项目“福建食用菌集聚产业科技服务体系建设示范——食用菌种质资源库建设、分类与认定前期技术研究”(项目编号:2003EAT20021)的部分研究内容
【文献出处】 食用菌学报,Acta Edulis Fungi,编辑部邮箱,2005年03期 【DOI】CNKI:SUN:SYJB.0.2005-03-001
