杨永彬; 林远崇; 兰家细; 杨淑云; 羿红
福建省蚕桑研究所; 福建省蚕桑研究所; 福建省蚕桑研究所; 福建省蚕桑研究所; 福建省蚕桑研究所 福建福州350003; 福建福州350003; 福建福州350003; 福建福州350003; 福建福州350003
【中文摘要】 根据真菌核糖体通用引物ITS1和ITS4扩增出13个福建袋栽香菇主要菌株的ITS、5.8s rDNA序列,将该序列提交NCBI中Genbank数据库,并根据该序列特征及ITS区的差异性,分别设计出针对菌株L9015和菌株Cr02进行PCR检测的特异引物探针,结果显示特异引物具有良好的特异性。
【英文摘要】 ITS(Internal-transcribed sequence) reigon has been often used to identify and characterize the microorganisms due to its high rate of mutation during the process of evolution.In this paper,the universe eukaryotic ribosome primers ITS1&ITS4 are used to amplify the ITS5.8s rDNA in the genome of thirteen Lentinula edodes strains,and then sequence these ITS5.8s rDNA sequences.After a careful analysis and comparison among them,two pairs of PCR primers(designated pair primer 1 and pair primer 2)are designed which can be served as the molecular marker to specificly amplify one piece of ITS5.8s rDNA from strain L9015 and strain Cr02.Result shows one piece of DNA(around 540bp in length and aonther(180bp) are specificly amplified from strain L9015 and strain Cr02 respecitively.
【中文关键词】 香菇; 菌株; ITS5.8srDNA; 特异引物
【英文关键词】 Lentinula edodes; Strain; ITS5.8srDNA; Specific PCR primer
【基金】福建省自然科学基金(B0510036)
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2008年02期 【DOI】CNKI:SUN:ZSYJ.0.2008-02-015
