郭学武; 姚丽敏; 龚建华; 肖冬光
天津科技大学天津市工业微生物重点实验室; 湖南省衡阳市船山实验中学; 中国科学院微生物研究所; 天津科技大学天津市工业微生物重点实验室 天津300222; 湖南421001; 北京100080; 天津300222
【中文摘要】 采集并组织分离到假褐云斑鹅膏(Amanita pseudoporphyria)和块鳞鹅膏(Amanita spissa)的纯培养菌丝。对2株鹅膏菌的培养发现虽然分离成功率很低,但是菌丝培养相对容易,在普通琼脂培养基上生长良好;2种鹅膏菌菌丝的生长形态和方式存在较大差别,对氨基酸的利用也存在差别,但是气生菌丝的延伸速率却非常相似。液体培养条件下,假褐云斑鹅膏菌丝体干重达到0.726mg·mL-1,块鳞鹅膏干重达到0.424mg·mL-1。
【英文摘要】 Sporophores of Amanita pseudoporphyria and Amanita spissa were collected and the pure cultures of them were obtained by way of tissue cultlure. Though there is little chance to obtain pure cultures of the two Amanita species, they were relatively easy to culture and well-grown mycelia were achieved on ordinary agar culture medium. There were great variances in extension morphology and growth pattern as well as capability in utilizing amino acids, but extensional rate of aerial hypha were very similar one another. In liquid culture system, the dry mycelial weight of A. pseudoporphyria reached 0.726mg·mL-1 and A. spissa reached 0.424mg·mL-1.
【中文关键词】 鹅膏菌; 外生菌根真菌; 纯培养菌丝; 液体培养
【英文关键词】 Amanita; Ectomycorrhiza; Liquid culture; Pure culture
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2008年03期 【DOI】CNKI:SUN:ZSYJ.0.2008-03-014
