胡乐琴; 潘迎捷
上海水产大学生命与技术学院; 上海水产大学食品学院 上海200090; 上海200090
【中文摘要】 概述了食用菌转化取得的进展和存在的问题,详细介绍了筛选标记、启动子、转化方法、整合方式以及将来的研究方向等方面的研究状况。筛选标记主要有营养缺陷型、药物抗性标记;启动子主要是同源启动子ras基因和gpd基因启动子;转化方法主要有PEG法、电击法、限制性内切酶介导法、农杆菌介导法,对比分析了各种方法的优缺点;重组方式主要是多位点整合;甲基化是导致转录水平低的一个原因;可能的提高转化率措施有采用强启动子、加入同源片段进行同源整合。
【英文摘要】 The progress and the problems in the transformation of edible fungi were summarized after introducing the select marker,the promoter,the ways of transformation,the pattern of integration and the future direction of the research in detail.Two main select markers were auxotroph and drug resistance.The promoter were major the ras gene of homologous promoter and the gpd gene promoter;the methods of transformation were main PEG method,electroporation method,restrictive enzyme-mediatedintegration and Agrobacterium-mediated method,which were advantages and disadvantages respectively after compared analyzing.The style of recombination is integration on multiple locus.The heavy methylation of the integrated DNA was one reason of low transcript.The possible measures which improved the frequency of transformation made homologous recombination by using strong promoter and adding homologous sequence.
【中文关键词】 食用菌; 转化; 育种; 分子技术
【英文关键词】 Edible fungi; Transformation; Breeding; Molecular-technology
【基金】上海市农委重点攻关项目资助
【文献出处】 中国食用菌,Edible Fungi of China,编辑部邮箱,2006年04期 【DOI】CNKI:SUN:ZSYJ.0.2006-04-001
