猪苓、伴生菌及蜜环菌共培养的形态学研究
MORPHOLOGICAL STUDIES ON DUAL AND TRIPLE CULTURE OF GRIFOLA UMBELLATA, AN UNNAMED COMPANION FUNGUS AND ARMILLARIELLA MELLEA
邢晓科 郭顺星
摘 要：本文对猪苓、伴生菌及蜜环菌两两共培养及三者共培养进行了宏观形态观察及细胞学水平上的研究.结果表明,猪苓与伴生菌共培养时,在二者之间形成一致密拮抗线,猪苓菌落表面菌丝分化产生大量菌丝束;猪苓与蜜环菌共培养时,猪苓能阻止蜜环菌菌索对其自身的进一步侵袭,互作区中的双方菌丝及菌索均停止生长;蜜环菌与伴生菌共培养时,蜜环菌能穿透整个伴生菌菌落,在伴生菌菌落下方产生大量分枝;三者共培养后,猪苓对蜜环菌的防御能力有所下降,伴生菌对蜜环菌的耐受力有所提高,蜜环菌产生的新分枝均向伴生菌一侧生长,猪苓与伴生菌之间并不形成致密拮抗线,只可见双方菌丝的白色交融区.猪苓与伴生菌均能在蜜环菌菌索皮层上形成侵入位点.
关键词：双重培养,三重培养,培养性状
分类号：Q939.5 文献标识码：A
文章编号：1007-3515(2003)04-0653-0660
基金项目：国家自然科学基金资助项目(no.39570012)
作者简介：郭顺星,通讯作者
作者单位：邢晓科（中国协和医科大学,中国医学科学院,药用植物研究所,北京,100094）
郭顺星（中国协和医科大学,中国医学科学院,药用植物研究所,北京,100094）
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